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Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
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Chapters
- 00:04Introduction
- 00:49Beam Collimation
- 01:32Spatial Overlap
- 02:34Electrooptical Modulation (EOM)
- 03:35Temporal Overlap
- 05:40Signal to Noise (SNR) Characterization, Frequency Axis Calibration, and Calibration of Spectral Resolution
- 07:23EOM1 Modulation and Epi-Mode SRS Imaging
- 08:53Results: Optimizing Spectral Resolution, Two-Color SRS, and False Two-Color H&E Staining of SRS Images
- 10:14Conclusion
Stimulated Raman scattering (SRS) microscopy is a powerful, nondestructive, and label-free imaging technique. One emerging application is stimulated Raman histology, where two-color SRS imaging at the protein and lipid Raman transitions are used to generate pseudo-hematoxylin and eosin images. Here, we demonstrate a protocol for real-time, two-color SRS imaging for tissue diagnosis.
Tags
Real-timeTwo-colorStimulated Raman ScatteringImagingMouse BrainSpectral FocusingSRS MicroscopyHistologySmall MoleculesDrugsCellsAchromatic LensesPump BeamLaser BeamDichroic MirrorSteering MirrorsScan MirrorsPhotodiodeLow Pass FiltersBeam SamplerFast PhotodiodeBand Pass FilterRF AttenuatorPhase Shifter
