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用于组织诊断的小鼠脑实时双色受激拉曼散射成像
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
DOI:

10:57 min

February 01, 2022

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Chapters

  • 00:04Introduction
  • 00:49Beam Collimation
  • 01:32Spatial Overlap
  • 02:34Electrooptical Modulation (EOM)
  • 03:35Temporal Overlap
  • 05:40Signal to Noise (SNR) Characterization, Frequency Axis Calibration, and Calibration of Spectral Resolution
  • 07:23EOM1 Modulation and Epi-Mode SRS Imaging
  • 08:53Results: Optimizing Spectral Resolution, Two-Color SRS, and False Two-Color H&E Staining of SRS Images
  • 10:14Conclusion

Summary

Automatic Translation

受激拉曼散射(SRS)显微镜是一种功能强大、无损且无标记的成像技术。一种新兴应用是刺激拉曼组织学,其中蛋白质和脂质拉曼转变处的双色SRS成像用于生成假苏木精和曙红图像。在这里,我们演示了用于组织诊断的实时双色SRS成像方案。

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