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Realtids, tofarvet stimuleret Raman-spredningsbilleddannelse af musehjerne til vævsdiagnose
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Chapters
- 00:04Introduction
- 00:49Beam Collimation
- 01:32Spatial Overlap
- 02:34Electrooptical Modulation (EOM)
- 03:35Temporal Overlap
- 05:40Signal to Noise (SNR) Characterization, Frequency Axis Calibration, and Calibration of Spectral Resolution
- 07:23EOM1 Modulation and Epi-Mode SRS Imaging
- 08:53Results: Optimizing Spectral Resolution, Two-Color SRS, and False Two-Color H&E Staining of SRS Images
- 10:14Conclusion
Stimuleret Raman scattering (SRS) mikroskopi er en kraftfuld, ikke-destruktiv og etiketfri billeddannelsesteknik. En ny applikation er stimuleret Raman histologi, hvor tofarvet SRS-billeddannelse ved protein- og lipid Raman-overgangene bruges til at generere pseudo-hæmatoxylin- og eosinbilleder. Her demonstrerer vi en protokol til realtids, tofarvet SRS-billeddannelse til vævsdiagnose.
Tags
Keywords: Real-timeTwo-colorStimulated Raman ScatteringImagingMouse BrainSpectral FocusingSRS MicroscopyHistologySmall MoleculesDrugsCellsAchromatic LensesPump BeamLaser BeamDichroic MirrorSteering MirrorsScan MirrorsPhotodiodeLow Pass FiltersBeam SamplerFast PhotodiodeBand Pass FilterRF AttenuatorPhase Shifter
