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Biology
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使用脉冲幅度调制叶绿素荧光法对作物中的非光化学猝灭进行高通量分析
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JoVE Journal
Biology
High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Please note that all translations are automatically generated.
Click here for the English version.
使用脉冲幅度调制叶绿素荧光法对作物中的非光化学猝灭进行高通量分析
DOI:
10.3791/63485-v
•
10:08 min
•
July 06, 2022
•
Dhananjay Gotarkar
2
,
Lynn Doran
2
,
Meghan Burns
2
,
Abigail Hinkle
,
Johannes Kromdijk
3
,
Steven J. Burgess
2
1
Carl R. Woese Institute for Genomic Biology
,
University of Illinois at Urbana-Champaign
,
2
Department of Plant Biology, Morrill Hall
,
University of Illinois at Urbana-Champaign
,
3
Environmental Plant Physiology group, Department of Plant Sciences
,
University of Cambridge
Chapters
00:04
Introduction
00:52
Collecting Leaf Samples from Field
02:11
Preparing Samples for Analysis
04:07
Measuring of Non-Photochemical Quenching Using Chlorophyll Fluorescence Imager
08:45
Results: High-Throughput Method for Measuring the Relaxation of NPQ in Field-Grown Soybeans
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议引入了一种高通量方法,用于通过脉冲幅度调制的叶绿素荧光测定法测量非光化学淬灭的松弛。该方法适用于田间生长的
甘氨酸max
,并且可以适应其他物种以筛选遗传多样性或繁殖种群。
Tags
Keywords: High-throughput Analysis
Non-photochemical Quenching
Pulse Amplitude Modulated Chlorophyll Fluorometry
Genetic Diversity
Soybean
Leaf Discs
24-well Plate
96-well Plate
Nasal Aspirator Filter
Humidity
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