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Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
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Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

DOI:
10.3791/63487-v

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09:55 min

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February 03, 2022

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, , , ,
1Brain-Immune Communication Lab,Institut Pasteur, 2Cytometry platform, CB UTechS,Institut Pasteur

Chapters

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

This study uses flow cytometry and two different gating strategies on isolated perfused mice brain choroid plexuses; this protocol identifies the main immune cell subsets that populate this brain structure.

Tags

Immune CellsChoroid PlexusesMouse ModelBrain RegulationCell DissectionCell IsolationCell CharacterizationCollagenase IVLateral VentricleThird VentricleFourth VentricleCell DissociationMACS BufferCell CentrifugationCell Washing

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