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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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JoVE Journal Chemistry
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

DOI:
10.3791/63503-v

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11:12 min

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February 07, 2022

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, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Chapters

  • 00:04Introduction
  • 00:55Rapid (Vial-Based) Protein Precipitation with Acetone
  • 02:27Protein Precipitation by Chloroform/Methanol/Water (CMW)
  • 04:30Protein Precipitation Using a Disposable Filtration Cartridge
  • 05:45Resolubilization of Protein Pellet with Formic Acid
  • 06:58Solid-Phase Extraction (SPE) Clean-Up
  • 08:20Results: Protein Precipitation via Vial-Based Versus Cartridge Approach
  • 10:22Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.

Tags

Keywords: Protein PrecipitationOrganic SolventsMass SpectrometrySample PreparationProteome PurificationAcetoneSodium ChlorideMethanolChloroformCentrifugationSDSPelletVortex

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