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实时检测和捕获共培养物中的侵袭细胞亚群

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Cancer Research

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JoVE Journal Cancer Research

Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures

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实时检测和捕获共培养物中的侵袭细胞亚群

DOI:

10.3791/63512-v

•

08:00 min

•

March 30, 2022

•

Ghada M. Sharif, Leon Der, Anna T. Riegel, Makarand Paranjape, Anton Wellstein

¹Lombardi Comprehensive Cancer Center,Georgetown University Medical Center, ²Department of Physics,Georgetown University

Chapters

00:05Introduction
00:49Cell Culture
01:17Cell Seeding and Assembly
04:09Impedance Measurement
04:44Detachment and Cell Collection
05:54Results: Detection and Capture of Invasive Cell Subpopulations in Real-Time
07:34Conclusion

Summary

Automatic Translation

我们描述了一种实时检测和捕获侵袭性细胞亚群的方法。实验设计使用实时细胞分析，通过监测细胞电阻抗的变化。可以捕获复杂组织中的浸润性癌症，免疫，内皮或基质细胞，并且可以评估共培养物的影响。

Tags

Real-time Detection Invasive Cell Subpopulations Co-cultures Cell Invasion Cell Motility Cell Culture Cell Analyzer Cell Recovery Cell Therapy Metastasis Cancer Cells Stromal Cells Immune Cells Heterogeneous Cell Mixes Trypsin EDTA Serum Cell Analysis Cell Migration

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