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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
 

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

Article DOI: 10.3791/63528-v • 06:59 min • June 22nd, 2022
June 22nd, 2022 •
1,3, 3, 2,4,5, 1,3,5
1Molecular Medicine, Hospital for Sick Children, 2Cell Biology, Hospital for Sick Children, 3Department of Physiology, University of Toronto, 4Department of Paediatrics, University of Toronto, 5Department of Biochemistry, University of Toronto

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Summary
Automatic Translation

This protocol describes a method for the study of electrogenic membrane proteins by measuring changes in membrane potential. This assay provides a platform for the functional readout of multiple ion channels endogenously expressed in epithelial cell lines.

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Keywords: Fluorescence-based Assay Membrane Potential High-throughput Ion Channels Epithelial Cells Calu-3 Caco-2 Cell Culture Trypsin 96-well Plate 384-well Plate Sodium-free Buffer Chloride-free Buffer NMDG
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