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Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots
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Developmental Biology
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JoVE Journal Developmental Biology
Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

DOI:
10.3791/63533-v

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05:51 min

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March 31, 2022

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, , , ,
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República (UdelaR), 2Laboratorio de Señalización Celular y Nanobiología,Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

Chapters

  • 00:04Introduction
  • 00:41Preparation of the Sample for Nanoindentation Experiments
  • 01:14Indentation Protocol
  • 03:16Results: Analysis of Force-Displacement Curves and Histograms
  • 05:13Conclusion

Summary

Automatic Translation

Automatic Translation

The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).

Tags

Keywords: Atomic Force MicroscopyEpidermal CellsArabidopsis RootsPhysical PropertiesCell WallIn VivoNon-invasiveSubcellular ResolutionSilicone GluePBSCantileverDeflection SensitivitySpring ConstantThermal TuneForce Curves

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