/
/
Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 01:06Biological Sample Preparation
- 01:37Force Spectroscopy Experimental Set-up and Acquisition
- 03:08Data Analysis
- 04:48Measure of Turgor Pressure
- 06:59Data Analysis
- 09:04Results: AFM Measurements of Cell Wall Mechanical Properties and Turgor Pressure Detection
- 10:35Conclusion
Here, we present atomic force microscopy (AFM), operated as a nano- and micro-indentation tool on cells and tissues. The instrument allows the simultaneous acquisition of 3D surface topography of the sample and its mechanical properties, including cell wall Young's modulus as well as turgor pressure.
