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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

DOI:
10.3791/63536-v

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09:20 min

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February 01, 2022

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,
1Department of Biological Sciences,University of Pittsburgh

Chapters

  • 00:04Introduction
  • 01:17Pre-Blocking of Samples to Prevent Early Digestion by MNase
  • 01:46Addition of Primary Antibody
  • 03:09Addition of pA-MNase or pA/G-MNase
  • 04:04Directed DNA Digestion
  • 05:04Sample Fractionation and DNA Extraction
  • 07:09Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

CUT&RUN and its variants can be used to determine protein occupancy on chromatin. This protocol describes how to determine protein localization on chromatin using single-cell uliCUT&RUN.

Tags

Keywords: Single-cellChromatinCUT&RUNProtein LocalizationLow-cellLow-abundanceAntibody96-wellMagnetic RackBlocking BufferWash BufferPrimary Antibody

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