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使用靶标下的超低输入切割在染色质上的单细胞因子定位,并使用核酸酶释放
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Biology
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JoVE Journal
Biology
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
Please note that all translations are automatically generated.
Click here for the English version.
使用靶标下的超低输入切割在染色质上的单细胞因子定位,并使用核酸酶释放
DOI:
10.3791/63536-v
•
09:20 min
•
February 01, 2022
•
Santana M. Lardo
,
Sarah J. Hainer
1
Department of Biological Sciences
,
University of Pittsburgh
Chapters
00:04
Introduction
01:17
Pre-Blocking of Samples to Prevent Early Digestion by MNase
01:46
Addition of Primary Antibody
03:09
Addition of pA-MNase or pA/G-MNase
04:04
Directed DNA Digestion
05:04
Sample Fractionation and DNA Extraction
07:09
Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
CUT&RUN及其变体可用于确定染色质上的蛋白质占用率。该协议描述了如何使用单细胞uliCUT&RUN确定染色质上的蛋白质定位。
Tags
Single-cell
Chromatin
CUT&RUN
Protein Localization
Low-cell
Low-abundance
Antibody
96-well
Magnetic Rack
Blocking Buffer
Wash Buffer
Primary Antibody
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