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Immunology and Infection

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JoVE Journal Immunology and Infection
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
 

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Summary
Automatic Translation

This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.

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Keywords: Co-culture Murine Small Intestine Epithelial Organoids Innate Lymphoid Cells In Vitro Model Intestinal Homeostasis Inflammation ILC1 CD44 Gastrointestinal Diseases Thermal Cross-linking Basal Extracellular Matrix Advanced DMEM F12 PBS2 Centrifugation
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