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A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

JoVE Journal
Biology

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JoVE Journal Biology

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

DOI:

10.3791/63578-v

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04:30 min

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March 04, 2022

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Heidi Stifter, Deborah E. Bauer

¹Neuroscience Department,Wellesley College

Chapters

00:04Introduction
00:34Prepare the Micro-Incinerator
01:02Sterilize the Pick, Spatula, or Scalpel
01:37Comparative Method: Sterilization of Instruments Using a Bunsen Burner
02:20Experiment
03:19Results: Bacterial Counts Across Sterilization Methods
04:03Conclusion

Summary

Automatic Translation

This article describes a method for sterilizing worm picks, spatulas, and scalpels using a micro-incinerator in place of an open flame.

Tags

Keywords: Flame-free Sterilization C. Elegans Picks Spatulas Scalpels Micro-incinerator Bunsen Burner Aseptic Technique OP50 E. Coli Bacterial Solution

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