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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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JoVE Journal Neuroscience
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

DOI:
10.3791/63614-v

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10:46 min

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May 13, 2022

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, ,
1Department of Biology, School of Science,Penn State Erie, The Behrend College, 2Magee Womens Research Institute—Allied Member

Chapters

  • 00:05Introduction
  • 00:31Collection and Concentration of Conditioned Cell Culture Media
  • 02:51Extracellular Vesicle (EV) Separation Using Size Exclusion Chromatography
  • 06:02Ultrafiltration of EV and Protein Fractions
  • 07:52Results: Separating EVs from the Conditioned Cell Culture Media Using Size Exclusion Chromatography
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol here demonstrates that extracellular vesicles can be adequately separated from conditioned cell culture media using size exclusion chromatography.

Tags

Keywords: Size Exclusion ChromatographyExtracellular VesiclesConditioned Cell Culture MediaSeparationCharacterizationIntracellular CommunicationUltracentrifugationFiltrationFraction Collection

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