A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Vocational School of Health Services/İstanbul,Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering,Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology,Istinye University, 43D Bioprinting Design & Prototyping R&D Center,Istinye University, 5Stem Cell and Tissue Engineering R&D Center,Istinye University, 6Medicine Faculty,Istinye University, 7Vocational School of Health Services,Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell),Liv Hospital
Chapters
- 00:04Introduction
- 00:56Culturing and Cryopreservation of Wharton’s Jelly Mesenchymal Stem Cells
- 01:30Production of Exosomes from Wharton’s Jelly Mesenchymal Stem Cells
- 02:20Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes
- 03:15Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs
- 05:07Conclusion
Her sigtede vi mod at opnå en formulering ved dopaminindlæsning af de isolerede exosomer af stamceller fra Whartons gelé mesenkymale stamceller. Exosomisolering og karakterisering, lægemiddelbelastning i de resulterende exosomer og den cytotoksiske aktivitet af den udviklede formulering er beskrevet i denne protokol.
