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使用光学镊子和散焦显微镜定量分析红细胞的粘弹性
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy
DOI:

08:03 min

March 25, 2022

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Sample Holders and Cell Cultures
  • 01:30OT‐Based Rheology Experiment and Analysis
  • 03:12Video and Contrast Image Acquisition
  • 05:40Soft Glassy Rheology Model and Experimental Analysis
  • 07:03Results: Characterizing RBC Surface from Values of K’ (ω), K” (ω), G’, and G”
  • 07:30Conclusion

Summary

Automatic Translation

本文描述了一种基于光学镊子和散焦显微镜的集成协议,以测量细胞的流变特性。该方案在研究不同生理病理条件下红细胞的粘弹性特性方面具有广泛的适用性。

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