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使用光学镊子和散焦显微镜定量分析红细胞的粘弹性
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Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用光学镊子和散焦显微镜定量分析红细胞的粘弹性
DOI:
10.3791/63626-v
•
08:03 min
•
March 25, 2022
•
Lucas Barreto*
1,2
,
Fran Gomez*
1,2
,
Pedro S. Lourenço
2
,
Douglas G. Freitas
2
,
Juliana Soares
3
,
Clemilson Berto-Junior
5
,
Ubirajara Agero
,
Nathan B. Viana
2
,
Bruno Pontes
2,3,7
1
Centro Nacional de Biologia Estrutural e Bioimagem – CENABIO
,
Universidade Federal do Rio de Janeiro
,
2
Instituto de Física, Programa de Pós-graduação Multidisciplinar em Física Aplicada
,
Universidade Federal do Rio de Janeiro
,
3
Instituto de Biofísica Carlos Chagas Filho, Programa de Pós-graduação em Ciências Biológicas Biofísica
,
Universidade Federal do Rio de Janeiro
,
4
Faculdade de Farmácia
,
Universidade Federal do Rio de Janeiro – Campus Macaé
,
5
Faculdade de Medicina, Programa de Pós-Graduação em Endocrinologia
,
Universidade Federal do Rio de Janeiro
,
6
Instituto de Ciências Exatas, Departamento de Física
,
Universidade Federal de Minas Gerais
,
7
Instituto de Ciências Biomédicas
,
Universidade Federal do Rio de Janeiro
Chapters
00:04
Introduction
00:43
Preparation of Sample Holders and Cell Cultures
01:30
OT‐Based Rheology Experiment and Analysis
03:12
Video and Contrast Image Acquisition
05:40
Soft Glassy Rheology Model and Experimental Analysis
07:03
Results: Characterizing RBC Surface from Values of K’ (ω), K” (ω), G’, and G”
07:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了一种基于光学镊子和散焦显微镜的集成协议,以测量细胞的流变特性。该方案在研究不同生理病理条件下红细胞的粘弹性特性方面具有广泛的适用性。
Tags
Optical Tweezers
Defocusing Microscopy
Viscoelastic Properties
Red Blood Cells
Erythrocytes
Single-cell Analysis
Mechanical Behavior
Form Factor
Stresses
Deformation
Polystyrene Spheres
Sinusoidal Function
Piezoelectric Stage
ImageJ Software
Center Of Mass
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