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Kvantitativ analyse af viskoelastiske egenskaber af røde blodlegemer ved hjælp af optisk pincet og defokuseringsmikroskopi
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy
DOI:

08:03 min

March 25, 2022

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Sample Holders and Cell Cultures
  • 01:30OT‐Based Rheology Experiment and Analysis
  • 03:12Video and Contrast Image Acquisition
  • 05:40Soft Glassy Rheology Model and Experimental Analysis
  • 07:03Results: Characterizing RBC Surface from Values of K’ (ω), K” (ω), G’, and G”
  • 07:30Conclusion

Summary

Automatic Translation

Her beskrives en integreret protokol baseret på optisk pincet og defokuseringsmikroskopi for at måle cellernes rheologiske egenskaber. Denne protokol har bred anvendelighed til at studere erytrocyternes viskoelastiske egenskaber under variable fysiopatologiske tilstande.

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