Journal
/
Biology
/
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
Please note that all translations are automatically generated. Click here for the English version.

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

DOI:
10.3791/63629-v

•

10:07 min

•

March 16, 2022

•

, , , , , , ,
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences,Henan University, 2Research Institute of Applied Biology,Shanxi University, 3School of Life Science,Shanxi University

Chapters

  • 00:04Introduction
  • 00:43Microinjection and Culture of the Eggs
  • 04:01Mutation Rate Estimation and Screening of Mutants
  • 06:08Establishment and Passaging of Mutant Lines and Egg Cryopreservation and Resuscitatio
  • 07:48Results: CRISPR/Cas9 Ribonuclease-Mediated Construction of Homozygous Locust Mutants
  • 09:19Conclusion

Summary

Automatic Translation

Automatic Translation

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Tags

Keywords: CRISPR/Cas9Homozygous MutantsMigratory LocustAgricultural PestGene EditingMicroinjectionEgg CollectionMutant ScreeningHomozygous MaintenanceRibonucleoproteinInjection NeedleEgg TanningInjection ParametersIncubation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63629?article_type=v

C. elegansのシナプス伝達の変異体の薬理学的特性評価のためのパラダイム
check_url/63629?article_type=v

タンパク質相互作用を発見したHaloTag技術を用いたタンパク質機能の特性評価
check_url/63629?article_type=v

CRISPR / Cas9経由哺乳動物細胞株におけるゲノム欠失の生成
check_url/63629?article_type=v

火花のサイレンシング:弱い電気魚のCRISPR/Cas9ゲノム編集
check_url/63629?article_type=v

CRISPR/Cas9を使用してヌル突然変異を生成するための Culex 蚊の胚の調製と注入
check_url/63629?article_type=v

CRISPR/Cas9を用いた初等ヒトB細胞のゲノム工学
check_url/63629?article_type=v

TGF β媒介性間葉転移(EndMT)およびCRISPR/Cas9遺伝子編集を用いたEndMTエフェクターの機能評価
check_url/63629?article_type=v

遺伝子治療アプリケーションのための造血幹細胞および前駆細胞のCRISPR/Cas9遺伝子編集
check_url/63629?article_type=v

オリエンタルショウジョウバエバクトセラ背側のCRISPR/Cas9変異誘発のためのプロトコル
check_url/63629?article_type=v

CRISPR-Cas9を介したゼブラフィッシュハートの正確なノックイン編集

Read Article