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Journal
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Neuroscience
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小鼠脑切片上淀粉样蛋白-β负荷的全区域与亚区域定量
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
Please note that all translations are automatically generated.
Click here for the English version.
小鼠脑切片上淀粉样蛋白-β负荷的全区域与亚区域定量
DOI:
10.3791/63669-v
•
07:28 min
•
May 19, 2022
•
Yuu Ohno
,
Riley Murphy
,
Matthew Choi
,
Weijun Ou
,
Rachita K. Sumbria
5
1
Henry E. Riggs School of Applied Life Sciences
,
Keck Graduate Institute
,
2
Crean College of Health and Behavioral Sciences
,
Chapman University
,
3
Keck Science Department
,
Claremont McKenna College
,
4
Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy
,
Chapman University
,
5
Department of Neurology
,
University of California, Irvine
Chapters
00:05
Introduction
00:42
Full-Region of Interest (ROI) Analysis
03:28
Sub-Region of Interest (ROI) Analysis
05:19
Results: Impact of Size of ROI on Aβ-Positive Area Quantification in the APP/PS1 Transgenic Mouse of Alzheimer’s Disease
06:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案描述并比较了对矢状小鼠脑切片进行全区域或子区域感兴趣分析的程序,以量化阿尔茨海默病的APP / PS1转基因小鼠模型中的淀粉样蛋白-β负荷。
Tags
Amyloid-beta
Plaque Load
APP/PS1 Transgenic Mice
Image Analysis
Full Region Of Interest
Sub-region Of Interest
Scale Bar
Brightness
Contrast
Hippocampus
Cortex
6-E10 Staining
Color Thresholding
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