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Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
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Bioengineering
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JoVE Journal Bioengineering
Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

DOI:
10.3791/63724-v

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10:56 min

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May 05, 2022

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, , , ,
1Emulate Inc. Boston, 2Center for Stem Cell and Organoid Medicine,Cincinnati Children’s Hospital Medical Center, 3Division of Pediatric General and Thoracic Surgery,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:05Introduction
  • 01:24Seeding of Organoid Fragments
  • 03:14Dynamic Culture of Intestine Chip
  • 04:19CYP450 Induction in the Intestine Chip
  • 05:49Sample Preparation for LC-MS Analysis
  • 07:15Stimulation with IFNγ
  • 08:24Results: CYP3A4 Induction and IFNγ Stimulation in the Intestine Chip
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

Biopsy-derived intestinal organoids and organ-on-a-chips technologies are combined into a microphysiological platform to recapitulate region-specific intestinal functionality.

Tags

Keywords: Human OrganoidsOrgan-on-a-chipIntestinal Region-specific FunctionalityIntestinal EpitheliumPharmacokineticsDrug-drug InteractionIntestinal Epithelial Barrier DysfunctionIntestinal PhysiologyPharmacodynamicsSeedingOrganoid FragmentsBMM DissociationOrganoid DigestionAdvanced DMEM F12Organoid Growth MediumCoated Chips

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