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Journal
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Biochemistry
/
啮齿动物组织的霰弹枪脂质组学
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Shotgun Lipidomics of Rodent Tissues
Please note that all translations are automatically generated.
Click here for the English version.
啮齿动物组织的霰弹枪脂质组学
DOI:
10.3791/63726-v
•
11:46 min
•
November 18, 2022
•
Oksana Lavrynenko
,
Sophie Dijon
,
Björn Titz
,
Nikolai V. Ivanov
1
PMI R&D
,
Philip Morris Products SA
Chapters
00:04
Introduction
00:42
Bradford Protein Determination Assay
01:39
BUME Extraction
05:16
MS Analysis
09:31
Results: Shotgun Lipidomics to Study the Effects of Cigarette Smoke on the Lungs of Mice
11:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
基于鸟枪式质谱的脂质组学可在各种啮齿动物组织的单次测量中同时提供广谱脂质类别的灵敏定量快照。
Tags
Keywords: Lipidomics
Lipid Quantification
Biomarkers
Toxicity
Lipid Classes
Ammonium Bicarbonate
Bovine Serum Albumin
Bradford Reagent
Internal Standards
Butanol-methanol
Heptane-ethyl Acetate
Liquid-liquid Extraction
Mass Spectrometry
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