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Microscopia a fluorescenza intravitale a campo largo della microcircolazione polmonare polmonare polmonare acuta sperimentale utilizzando un sistema di imaging stabilizzato sotto vuoto
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1Department of Physiology and Biophysics,Dalhousie University, 2Department of Microbiology and Immunology,Dalhousie University, 3Department of Neuroscience,Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine,Dalhousie University, 5Live Cell Imaging Center,University of Calgary, 6Department of Physiology and Pharmacology,University of Calgary, 7Department of Biochemistry and Molecular Biology,University of Calgary, 8Department of Pharmacology,Dalhousie University
Chapters
- 00:04Introduction
- 00:36Preparation of the Vacuum-Stabilized Lung Imaging System
- 01:10Intubation
- 01:57Ventilation
- 02:47Thoracotomy
- 05:10Microscopy
- 06:56Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
- 08:48Conclusion
La microscopia a fluorescenza intravitale può essere utilizzata per studiare le interazioni leucocita-endoteliali e la perfusione capillare in tempo reale. Questo protocollo descrive i metodi per visualizzare e quantificare questi parametri nel microcircolo polmonare utilizzando un sistema di imaging polmonare stabilizzato nel vuoto.
