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Isolering, kultur og karakterisering af primære Schwann-celler, keratinocytter og fibroblaster fra human forhud
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
DOI:

10:36 min

March 23, 2022

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Chapters

  • 00:04Introduction
  • 01:17Epidermis and Dermis Separation Procedure
  • 03:03Keratinocyte Isolation from the Epidermis
  • 05:02Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis
  • 08:18Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts
  • 09:43Conclusion

Summary

Automatic Translation

Undersøgelsen af sårheling forbundet med muskuloskeletal skade kræver ofte vurdering af in vitro-interaktioner mellem Schwann-celler (SC'er), keratinocytter og fibroblaster. Denne protokol beskriver isolering, dyrkning og karakterisering af disse primære celler fra den menneskelige forhud.

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