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Dissection Techniques and Histological Sampling of the Heart in Large Animal Models for Cardiovascular Diseases
JoVE Journal
Medicine
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JoVE Journal Medicine
Dissection Techniques and Histological Sampling of the Heart in Large Animal Models for Cardiovascular Diseases
DOI:

10:53 min

June 16, 2022

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Chapters

  • 00:04Introduction
  • 00:59Necropsy Protocol
  • 04:16Dissection Protocol
  • 06:21Sampling Protocol for Histology
  • 07:53Sampling of Coronary Arteries
  • 08:40The Four Chambers/Echocardiographic Plane Dissection Technique of the Heart
  • 09:46Results: Visualization and Histological Examination of the Heart
  • 10:13Conclusion

Summary

Automatic Translation

Due to the complex anatomy, a consistently reproducible dissection and harvesting protocol for cardiac samples can be challenging to implement. This manuscript presents the key elements of some standard cardiac dissection protocols, highlighting both the gross examination approaches and the sampling sites commonly used for histopathologic examination.

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