Journal
/
Bioengineering
/
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

DOI:
10.3791/63821-v

•

10:23 min

•

November 04, 2022

•

,
1Department of Biomedical Engineering, Pratt School of Engineering,Duke University, 2Department of Medicine, Division of Nephrology,Duke University School of Medicine, 3Department of Cell Biology,Duke University, 4Center for Biomolecular and Tissue Engineering,Duke University

Chapters

  • 00:04Introduction
  • 00:59Preparation of Microfluidic Organ Chip Devices
  • 01:31Seeding of viECs and Intermediate Mesoderm Cells into the Microfluidic Device
  • 07:15Podocyte Induction and Chip Maintenance
  • 08:03Results: Differentiation of viECs and Podocytes on Organ-On-A-Chip Device
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to engineer a personalized organ-on-a-chip system that recapitulates the structure and function of the kidney glomerular filtration barrier by integrating genetically matched epithelial and vascular endothelial cells differentiated from human induced pluripotent stem cells. This bioengineered system can advance kidney precision medicine and related applications.

Tags

Keywords: Isogenic Glomerulus ChipHuman Induced Pluripotent Stem CellsIPSCGlomerular EpitheliumGlomerular EndotheliumPatient-specific Disease ModelingNephrotoxicity TestingPrecision MedicineDrug ScreeningMechanistic StudiesBody-on-a-chipPatient-specific ResponsesBasement Membrane MatrixVascular Endothelial CellsMicrofluidic ChipPDMS Membrane

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63821?article_type=v

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
check_url/63821?article_type=v

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
check_url/63821?article_type=v

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
check_url/63821?article_type=v

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
check_url/63821?article_type=v

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
check_url/63821?article_type=v

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
check_url/63821?article_type=v

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
check_url/63821?article_type=v

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
check_url/63821?article_type=v

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
check_url/63821?article_type=v

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Read Article