Journal
/
Biology
/
从田间收集的蚊子中分离出蚊相关病毒
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Please note that all translations are automatically generated. Click here for the English version.

从田间收集的蚊子中分离出蚊相关病毒

DOI:
10.3791/63852-v

•

06:41 min

•

August 31, 2022

•

, , , , , , ,
1Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

Chapters

  • 00:04Introduction
  • 01:17Mosquito Sampling, Sorting, and Grinding
  • 02:15Preparation of Cells and Cell Culture Maintenance Medium
  • 02:56Virus Isolation
  • 04:38Results: Isolation and Amplification of Viruses Using Vertebrate and Mosquito Cell Lines
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

由于测序技术的广泛使用，在蚊子中发现了许多新的病毒样序列。我们提供了一种使用脊椎动物和蚊子细胞系分离和扩增病毒的有效程序，这可能作为未来研究蚊子相关病毒（包括蚊媒和蚊子特异性病毒）的基础。

Tags

Keywords: Virus IsolationMosquito-associated VirusesMosquito-borne DiseasesVirus AmplificationAedes AlbopictusRNAi-deficient Mosquito Cell LineBHK-21 Cell LineCell Culture Maintenance MediumContamination PreventionCentrifugationSupernatantBiosafetyAnimal Experimentation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63852?article_type=v

从AE肠和唾液腺的解剖。埃及伊蚊蚊
check_url/63852?article_type=v

骨骼肌的线粒体分离
check_url/63852?article_type=v

kinesin的分离和纯化果蝇胚胎
check_url/63852?article_type=v

在EMBL的MultiBac蛋白质复杂的生产平台
check_url/63852?article_type=v

小非编码RNA从人血清中分离
check_url/63852?article_type=v

一种多检测试验为疟疾蚊子发射
check_url/63852?article_type=v

新型杀虫化学对蚊子毒性测试规程
check_url/63852?article_type=v

强迫流涎法分析蚊虫的载体能力
check_url/63852?article_type=v

分离高质量鼠心房和心室肌细胞，同时测量 Ca2+ 瞬变和 L 型钙电流
check_url/63852?article_type=v

从冷冻嵌合肝组织中提取染色质，用于染色质免疫沉淀分析

Read Article