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Neuroscience
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年轻成人和老年沙鼠耳蜗的免疫标记和计数带状突触
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
Please note that all translations are automatically generated.
Click here for the English version.
年轻成人和老年沙鼠耳蜗的免疫标记和计数带状突触
DOI:
10.3791/63874-v
•
08:25 min
•
April 21, 2022
•
Friederike Steenken
,
Sonny Bovee
,
Christine Köppl
1
Cluster of Excellence “Hearing4all” and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science
,
Carl von Ossietzky University Oldenburg
Chapters
00:05
Introduction
01:04
Tissue Preparation
02:21
Fine Dissection of the Cochlea
04:39
Synapse Quantification
06:52
Results: Immunostaining of the Processed Cochleae
07:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种通过免疫标记传入突触结构和毛细胞,在老年组织中淬灭自发荧光,解剖和估计耳蜗的长度以及量化通过共聚焦成像获得的图像堆栈中的突触来处理年轻成人和老年沙鼠耳蜗的方案。
Tags
Immunolabeling
Ribbon Synapses
Gerbil Cochlea
Aging
Synaptopathy
Cochlear Dissection
Autofluorescence Quencher
Cochlear Half
Razor Blade
Spring Scissors
Forceps
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