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Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
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Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

DOI:
10.3791/63918-v

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09:47 min

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August 12, 2022

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, , ,
1Department of Biomedical Sciences, College of Graduate Studies,Midwestern University, 2Department of Child Health, College of Medicine-Phoenix,University of Arizona, Phoenix

Chapters

  • 00:05Introduction
  • 00:56Myograph Unit Preparation
  • 02:03Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
  • 03:24Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
  • 05:18Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
  • 06:25Contribution of the Endothelial Layer to Aortic Vasorelaxation
  • 08:00Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the concepts and technical application of the tensometric myograph technique using a multi-chamber myograph system in the experimental ex vivo assessment of mouse aortic endothelial function.

Tags

Keywords: Tensometric MyographyEndothelium-dependent VasorelaxationMouse Thoracic AortaHEPES-PSS SolutionHigh-potassium SolutionCarbogen GasAortic DissectionAortic SegmentsMyograph UnitData Acquisition

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