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Måling av endotelavhengig vasorelaxasjon i musens thoraxaorta ved bruk av tensometrisk småvolumkammermyografi
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
DOI:

09:47 min

August 12, 2022

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Chapters

  • 00:05Introduction
  • 00:56Myograph Unit Preparation
  • 02:03Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
  • 03:24Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
  • 05:18Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
  • 06:25Contribution of the Endothelial Layer to Aortic Vasorelaxation
  • 08:00Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
  • 09:06Conclusion

Summary

Automatic Translation

Denne protokollen beskriver konseptene og den tekniske anvendelsen av den tensometriske myografteknikken ved hjelp av et multikammermyografsystem i den eksperimentelle ex vivo-vurderingen av musens aorta-endotelfunksjon.

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