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Utilizando a fatia pulmonar cortada com precisão para estudar a regulação contratil das vias aéreas e do músculo liso arterial intrapulmonar
JoVE Journal
Biology
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JoVE Journal Biology
Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
DOI:

08:59 min

May 05, 2022

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Chapters

  • 00:04Introduction
  • 00:47Inflation of Mouse Lungs
  • 03:25Sectioning of Lung Lobes
  • 04:09Analyzing Contractile Responses of Intrapulmonary Airways and Arteries
  • 05:08Analyzing Ca2+ Signaling of Airway or Vascular Smooth Muscle Cells
  • 06:45Results: Analyses of Airways and Pulmonary Arteries in Mouse Precision-Cut Lung Slices
  • 08:07Conclusion

Summary

Automatic Translation

O presente protocolo descreve a preparação e utilização de fatias pulmonares cortadas com precisão do rato para avaliar as vias aéreas e a contratilidade muscular liso arterial intrapulmonar em um ambiente quase in vivo .

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