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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

DOI:
10.3791/63953-v

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06:49 min

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May 24, 2022

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,
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences,United Arab Emirates University, 2Zayed Center for Health Sciences,United Arab Emirates University

Chapters

  • 00:04Introduction
  • 00:28Transfection of HEK293T Cells Using Either Polymer Polyethylenimine (PEI) or Lipofectamine 3000 Reagent
  • 02:48Lentiviral Vectors (LVS) Collection and Ultracentrifugation
  • 05:07Results: Lentiviral Mediated Delivery of shRNAs in hESCs and NPCs Using Cationic Polymer PEI
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.

Tags

Keywords: LentiviralShRNAHEK293TTransfectionPolyethyleniminePEICationic PolymerLow Protein Binding FilterSucrose CushionUltracentrifugationDMEMFBSPenicillin StreptomycinConfluencyPlasmid DNAPackagingLipofectamineVirus-containing MediaCentrifugation

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