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Chapters
- 00:04Introduction
- 01:00Transforming cDNA Library into the QuerySchizosaccharomyces pombe Mutant Strain and Screening Multicopy Suppressors
- 05:16Validating the Suppressors of the Phenotype Associated with the Query Mutant Strain
- 07:41Isolation of the Plasmid from the Schizosaccharomyces pombeSuppressor Clones
- 09:55Identification of the Gene Encoded by the Suppressor Clone
- 10:48Results: Screening Multicopy Suppressor of ell1 Deletion-Associated Genotoxic Stress Sensitivity
- 12:49Conclusion
This work describes a protocol for a multicopy suppressor genetic screen in Schizosaccharomyces pombe. This screen uses a genome-wide plasmid library to identify suppressor clone(s) of a loss-of-function phenotype associated with a query mutant strain. Novel genetic suppressors of the ell1 null mutant were identified using this screen.
