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지지된 지질 이중층에 대한 막으로 묶인 최소 액틴 피질의 재구성
JoVE Journal
Biology
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JoVE Journal
Biology
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Please note that all translations are automatically generated.
Click here for the English version.
지지된 지질 이중층에 대한 막으로 묶인 최소 액틴 피질의 재구성
DOI:
10.3791/63968-v
•
11:55 min
•
July 12, 2022
•
Darius Vasco Köster
,
Abrar Bhat
,
Sankarshan Talluri
,
Satyajit Mayor
1
Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School
,
University of Warwick
,
2
National Centre for Biological Sciences
,
Tata Institute of Fundamental Research
Chapters
00:05
Introduction
00:45
Preparation of Sample Chambers
02:59
Preparation of Supported Lipid Bilayers
04:31
Addition of Membrane-Actin Linker
05:27
Quality Assessment of the Lipid Bilayer
07:06
Polymerization and Addition of Fluorescent Actin Filaments
09:02
Addition of Myosin II
10:21
Results: Understanding the Membrane-Cortex Interface of Cells
11:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
이 프로토콜은 형광 현미경을 사용하여 재구성된 막 연결 세포골격 네트워크의 역학을 연구하기 위해 지지된 지질 이중층의 형성과 세포골격 필라멘트 및 운동 단백질의 추가를 설명합니다.
Tags
Membrane-tethered Actin Cortices
Supported Lipid Bilayers
Cytoskeletal Proteins
Reconstitution
Light Microscopy
Protein Assembly
Lipid Bilayer Formation
UV Curable Adhesive
Chamber Fabrication
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