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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Cancer Research
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JoVE Journal Cancer Research
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

DOI:
10.3791/63973-v

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08:56 min

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July 12, 2022

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, , , , , ,
1Johannes Kepler University Linz, 2Charité – University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Chapters

  • 00:05Introduction
  • 00:55Stain DLBCL Cells with Different Senescence Markers
  • 04:01Imaging Senescence Markers Using the Imaging Flow Cytometer System
  • 07:12Results: Visualization and Quantification of Senescent Cells on a Single-Cell Level
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.

Tags

Keywords: Simultaneous ImagingFlow CytometryFluorescent Senescence MarkersTherapy-induced Senescent Cancer CellsDLBCL Cell LinesEdUChloroquineC12 FDG2-phenylethyl-beta-d-thiogalactosideParaformaldehyde FixationSaponin Permeabilization

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