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1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Chapters
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Cerebrale organoider giver hidtil usete muligheder for at studere organudvikling og human sygdomspatologi. Selvom der er opnået stor succes med cerebrale organoidkultursystemer, er der stadig operationelle vanskeligheder med at anvende denne teknologi. Den nuværende protokol beskriver en cerebral organoid procedure, der letter medium forandring og organoid overførsel.
