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Aislamiento y análisis de vesículas extracelulares trazables y funcionalizadas a partir del plasma y tejidos sólidos
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
DOI:

09:57 min

October 17, 2022

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Chapters

  • 00:05Introduction
  • 01:20Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
  • 02:50Origin Analysis of Extracellular Vesicles
  • 05:17Protein Content Analysis of Extracellular Vesicles
  • 08:06Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
  • 09:13Conclusion

Summary

Automatic Translation

El presente protocolo describe un método para extraer vesículas extracelulares de la sangre periférica y tejidos sólidos con el posterior perfil de antígenos de superficie y cargas de proteínas.

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