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用解剖学特异性单细胞基因表达方法研究成瘾行为中抗奖励的驱动因素

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods

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用解剖学特异性单细胞基因表达方法研究成瘾行为中抗奖励的驱动因素

DOI:

10.3791/64014-v

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09:29 min

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August 04, 2022

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Sean J. O’Sullivan*^1,2,3, Ankita Srivastava*¹, Rajanikanth Vadigepalli, James S. Schwaber

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, ²Sidney Kimmel Medical College,Thomas Jefferson University, ³Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine

Chapters

00:04Introduction
00:32Running qPCR Chip on a Microfluidic RT‐qPCR Platform
02:46Data Analysis
06:48Results: Analyzing GLP‐1 Enriched Neuronal Samples
08:51Conclusion

Summary

Automatic Translation

激光捕获显微切割和微流体RT-qPCR的组合为测量单个神经元和神经胶质细胞中的转录组提供了解剖学和生物技术特异性。将创造性方法与系统生物学方法应用于精神疾病可能会导致理解和治疗的突破，例如成瘾中的神经炎症抗奖励假说。

Tags

Single-cell Gene Expression Anatomically Specific Antireward Addiction Behavior Microfluidic RT-qPCR Gene Expression Profiling Spatial Resolution Molecular Specificity MRNA Preparation QPCR Chip Microfluidic Mixing Device Data Analysis Software Sample Plate Setup Thermal Cycling Program

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