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使用福尔马林固定和石蜡包埋组织样品的质谱成像扩展肿瘤微环境的理解
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JoVE Journal Cancer Research
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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使用福尔马林固定和石蜡包埋组织样品的质谱成像扩展肿瘤微环境的理解

DOI:
10.3791/64015-v

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06:47 min

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June 29, 2022

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, , ,
1Department of Translational Molecular Pathology,The University of Texas MD Anderson Cancer Center

Chapters

  • 00:04Introduction
  • 01:06Antibody Panel Preparation and Antibody Staining
  • 02:35Image Acquisition
  • 04:31Results: Isotopic Purity and Channel Cross-Talk, Staining Images in Different Tissues, and Images of the Staining Before and After Image Preparation
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

在癌症免疫治疗时代，人们对阐明肿瘤微环境动力学的兴趣惊人地增加。该协议详细介绍了质谱成像技术及其染色和成像步骤，允许高度多路复用的空间分析。

Tags

Mass Spectrometry ImagingImmune Cell Characterization40-marker PanelAntibody OptimizationSingle Cell ResolutionMarker Co-expressionSpatial Analysis

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