Journal
/
/
시험관 내에서 파골세포 재흡수를 시각화하고 정량화하는 간단한 구덩이 분석 프로토콜
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
DOI:

07:03 min

June 16, 2022

, , , ,

Chapters

  • 00:04Introduction
  • 00:51Calcification of 96-Well Cell Culture Plates
  • 01:54Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
  • 02:49Expansion of Osteoclasts Progenitors and Induction of Osteoclastogenesis
  • 04:19Fluorescence Staining of Osteoclasts and Calcium Phosphate Coating
  • 05:17Results: Analysis of Mature Osteoclast Cultured on Calcium Phosphate Coated Wells
  • 06:38Conclusion

Summary

Automatic Translation

여기에서, 우리는 인산칼슘 코팅 세포 배양 플레이트를 이용한 재흡수 구덩이 분석을 위한 간단하고 효과적인 분석 절차를 제시한다.

Related Videos

Read Article