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Chapters
- 00:04Introduction
- 01:00Setup of the nFCM Instrument and Measurements of the nFCM Standards
- 03:47Determining the Particle Concentration of an Unlabeled Sample and Generating a PDF Report
- 07:50nFCM Analysis: PDF Generation for Subpopulation Analysis
- 09:26Results: Tetraspanin Presentation on SW620 Derived EVs and C2C12 Derived EVs
- 12:00Conclusion
The latest generation of EV characterization tools are capable of single EV analysis across multiple parameters simultaneously. Nano-flow cytometry measures all biological particles larger than 45 nm without labeling and identifies specific characteristics of subpopulations by a variety of fluorescent labeling techniques.
Tags
Keywords:
Single Extracellular VesicleTransmembrane ProteinNano-flow CytometryEV CharacterizationParticle AnalysisLabel-free DetectionFluorescence DetectionVesicle SubpopulationsPlasmaCSFCell CultureVirusesLipid NanoparticlesNanomaterialsLabeling ProtocolQC BeadsPressure ControlSample DilutionCleaning SolutionSize Standard Beads
