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通过纳流式细胞术表征单个细胞外囊泡跨膜蛋白
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
通过纳流式细胞术表征单个细胞外囊泡跨膜蛋白
DOI:
10.3791/64020-v
•
12:27 min
•
July 26, 2022
•
Rebecca Lees*
1
,
Robert Tempest*
1
,
Alice Law*
1
,
Dimitri Aubert*
1
,
Owen G. Davies*
2
,
Soraya Williams*
2
,
Nick Peake*
3
,
Ben Peacock*
1
1
NanoFCM Co., Ltd
,
2
School of Sport, Exercise and Health Sciences
,
Loughborough University
,
3
Biomolecular Sciences Research Centre
,
Sheffield Hallam University
Chapters
00:04
Introduction
01:00
Setup of the nFCM Instrument and Measurements of the nFCM Standards
03:47
Determining the Particle Concentration of an Unlabeled Sample and Generating a PDF Report
07:50
nFCM Analysis: PDF Generation for Subpopulation Analysis
09:26
Results: Tetraspanin Presentation on SW620 Derived EVs and C2C12 Derived EVs
12:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
最新一代的电动汽车表征工具能够同时对多个参数进行单个电动汽车分析。纳米流式细胞术无需标记即可测量所有大于 45 nm 的生物颗粒,并通过各种荧光标记技术识别亚群的特定特征。
Tags
Single Extracellular Vesicle
Transmembrane Protein
Nano-flow Cytometry
EV Characterization
Particle Analysis
Label-free Detection
Fluorescence Detection
Vesicle Subpopulations
Plasma
CSF
Cell Culture
Viruses
Lipid Nanoparticles
Nanomaterials
Labeling Protocol
QC Beads
Pressure Control
Sample Dilution
Cleaning Solution
Size Standard Beads
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