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通过纳流式细胞术表征单个细胞外囊泡跨膜蛋白
JoVE Journal
Biology
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JoVE Journal Biology
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
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通过纳流式细胞术表征单个细胞外囊泡跨膜蛋白

DOI:
10.3791/64020-v

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12:27 min

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July 26, 2022

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, , , , , , ,
1NanoFCM Co., Ltd, 2School of Sport, Exercise and Health Sciences,Loughborough University, 3Biomolecular Sciences Research Centre,Sheffield Hallam University

Chapters

  • 00:04Introduction
  • 01:00Setup of the nFCM Instrument and Measurements of the nFCM Standards
  • 03:47Determining the Particle Concentration of an Unlabeled Sample and Generating a PDF Report
  • 07:50nFCM Analysis: PDF Generation for Subpopulation Analysis
  • 09:26Results: Tetraspanin Presentation on SW620 Derived EVs and C2C12 Derived EVs
  • 12:00Conclusion

Summary

Automatic Translation

Automatic Translation

最新一代的电动汽车表征工具能够同时对多个参数进行单个电动汽车分析。纳米流式细胞术无需标记即可测量所有大于 45 nm 的生物颗粒，并通过各种荧光标记技术识别亚群的特定特征。

Tags

Single Extracellular VesicleTransmembrane ProteinNano-flow CytometryEV CharacterizationParticle AnalysisLabel-free DetectionFluorescence DetectionVesicle SubpopulationsPlasmaCSFCell CultureVirusesLipid NanoparticlesNanomaterialsLabeling ProtocolQC BeadsPressure ControlSample DilutionCleaning SolutionSize Standard Beads

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