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突触膜的微移植以重新激活人突触受体以进行功能研究
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
DOI:

10:08 min

July 20, 2022

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Chapters

  • 00:04Introduction
  • 01:13Loading the Sample
  • 02:06Injecting the Oocytes
  • 02:49Recording of Ion Currents Using a Two-Electrode Voltage Clamp
  • 06:50Analyzing TEVC Recordings
  • 07:30Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
  • 09:25Conclusion

Summary

Automatic Translation

该方案表明,通过将突触膜微移植到 非洲爪蟾 卵母细胞中,可以记录α-氨基-3-羟基-5-甲基-4-异噁唑丙酸和γ-氨基丁酸受体的一致和可靠的反应。

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