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Neuroscience
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突触膜的微移植以重新激活人突触受体以进行功能研究
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
Please note that all translations are automatically generated.
Click here for the English version.
突触膜的微移植以重新激活人突触受体以进行功能研究
DOI:
10.3791/64024-v
•
10:08 min
•
July 20, 2022
•
Brice Miller*
1
,
Ashli Powell*
1
,
Berenice A. Gutierrez
,
Agenor Limon
1
The Mitchell Center for Neurodegenerative Diseases, Department of Neurology
,
University of Texas Medical Branch
Chapters
00:04
Introduction
01:13
Loading the Sample
02:06
Injecting the Oocytes
02:49
Recording of Ion Currents Using a Two-Electrode Voltage Clamp
06:50
Analyzing TEVC Recordings
07:30
Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
09:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案表明,通过将突触膜微移植到
非洲爪蟾
卵母细胞中,可以记录α-氨基-3-羟基-5-甲基-4-异噁唑丙酸和γ-氨基丁酸受体的一致和可靠的反应。
Tags
Microtransplantation
Synaptic Membranes
Synaptic Receptors
Functional Studies
Neurodegenerative Disorders
Mental Health Disorders
Synaptic Communication
Synaptic Receptors
Brain Receptors
Receptor Functionality
Receptor Changes
Receptor Activity
Injection
Nanoinjector
Oocytes
Sample Preparation
Sonication
Thermoplastic
Manipulator
Foot Pedal
Petri Dish
Control Group
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