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Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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Biochemistry
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JoVE Journal Biochemistry
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

DOI:
10.3791/64061-v

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07:59 min

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August 17, 2022

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, , , ,
1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Chapters

  • 00:04Introduction
  • 00:58Preparation of NCP-DNA Repair Factor Complex
  • 02:31Freezing Nucleosomal Complex
  • 04:25Screen Grids
  • 06:02Results: Preparation of Nucleosomal Complexes Using Frozen TEM Grids
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines in detail the preparation of nucleosomal complexes using two methods of sample preparation for freezing TEM grids.

Tags

Keywords: NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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