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清醒小鼠小胶质细胞动力学和神经元活动的同时成像
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
Please note that all translations are automatically generated.
Click here for the English version.
清醒小鼠小胶质细胞动力学和神经元活动的同时成像
DOI:
10.3791/64111-v
•
08:26 min
•
August 23, 2022
•
Hisato Maruoka
,
Ryosuke Kamei
,
Shunsuke Mizutani
,
Qingrui Liu
,
Shigeo Okabe
1
Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine
,
The University of Tokyo
Chapters
00:04
Introduction
01:00
AAV Injection
03:04
Cranial Window Implantation
04:36
In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
06:42
Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
07:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种将腺相关病毒注射与颅窗植入相结合的方案,用于同时对清醒小鼠的小胶质细胞动力学和神经元活动进行成像。
Tags
Simultaneous Imaging
Microglia Dynamics
Neuronal Activity
Awake Mice
AAV Injection
Cranial Window
Stereotaxic Instrument
Motion Artifact
Primary Visual Cortex
Microinjector
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