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体外选择核酸适配体以区分感染性和非感染性病毒
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Bioengineering
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JoVE Journal Bioengineering
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
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体外选择核酸适配体以区分感染性和非感染性病毒

DOI:
10.3791/64127-v

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12:23 min

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September 07, 2022

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, , ,
1INQUIMAE (CONICET), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires, 2Department of Chemistry,University of Illinois at Urbana-Champaign, 3Department of Chemistry,University of Texas at Austin

Chapters

  • 00:04Introduction
  • 01:05Design and Synthesis of DNA Library and Primers and Infectious and Non‐Infectious Virus Samples
  • 01:46In Vitro Selection or SELEX Process: Initial Round
  • 06:38Subsequent Selection Rounds
  • 07:55Monitoring the SELEX Process
  • 08:46High‐Throughput Sequencing, Sequencing Analysis, and Aptamer Binding Validation and Assays
  • 10:38Results: SELEX Process of Aptamers for Distinguishing Infectious from Non‐Infectious Viruses
  • 11:42Conclusion

Summary

Automatic Translation

Automatic Translation

我们提供的方案通常可以应用于选择仅与感染性病毒结合的适配体，而不应用于通过消毒方法或任何其他类似病毒变得非传染性的病毒。这为在便携式和快速检测中确定感染性状态提供了可能性。

Tags

Aptamer SelectionIn Vitro SelectionInfectious VirusNon-infectious VirusSELEXAffinity-based SelectionVirus DetectionVirus InfectivityDiagnostic SensingEnvironmental Monitoring

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