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JoVE Journal Cancer Research
Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
 

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Article DOI: 10.3791/64146-v • 08:02 min • July 27th, 2022
July 27th, 2022 •
1, 1,2, 1,3, 1
1Biology Department, Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology, New York University, 3Enveda Therapeutics India Pvt Ltd.

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Summary
Automatic Translation

The present protocol describes the setting up of 3D 'on top' cultures of a non-transformed breast epithelial cell line, MCF10A, that has been modified to study Platelet Activating Factor (PAF) induced transformation. Immuno-fluorescence has been used to assess the transformation and is discussed in detail.

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Keywords: 3D Cell Culture LrECM Phospholipid Mediator PAF Breast Cancer Biomarkers Drug Targets Tumorigenesis Morphogenesis
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