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生理尺寸微流控培养装置中上皮细胞单层的制备和结构评价
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation and Structural Evaluation of Epithelial Cell Monolayers in a Physiologically Sized Microfluidic Culture Device
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生理尺寸微流控培养装置中上皮细胞单层的制备和结构评价

DOI:
10.3791/64148-v

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07:38 min

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July 01, 2022

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, , ,
1Department of Biomedical Engineering, School of Science and Engineering,Tulane University

Chapters

  • 00:04Introduction
  • 00:48Microfluidic Channel Fabrication and Pre‐Treatment
  • 01:49Cell Culture in the Microfluidic Channel
  • 02:35Cell Fixation
  • 04:11Cell Staining and Mounting
  • 05:25Cell Imaging with Confocal Microscopy
  • 05:55Results: Culture Duration Effects on Monolayer Production
  • 06:52Conclusion

Summary

Automatic Translation

Automatic Translation

所提出的协议描述了基于鬼笔环肽的丝状肌动蛋白染色技术的开发和使用，该技术与共聚焦激光扫描显微镜（CLSM）一起可视化微流体动态培养通道和传统固定孔静态培养室中的贴壁细胞层结构。这种方法有助于评估细胞层融合度、单层形成和层厚均匀性。

Tags

Epithelial Cell MonolayersMicrofluidic Culture DeviceAlveolar EpitheliumRespiratory Distress SyndromeVentilator-induced Lung InjuryPoly-d-lysineFibronectinNCI H441 CellsRPMI 1640 MediumFormaldehydeDPBSCell CultureCell Characterization

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