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从成年非洲绿松石鳉鱼的脑组织中温和分离细胞核,这是一种自然短暂的衰老研究模型
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
DOI:

09:28 min

August 09, 2022

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Chapters

  • 00:05Introduction
  • 01:07Nuclei Isolation
  • 04:46Quantify Singlet Nuclei, Debris, and Multiplet Proportion by Flow Cytometry
  • 07:35Results: Nuclei Quantification and Purity Assessment by Flow Cytometry
  • 08:37Conclusion

Summary

Automatic Translation

在这里,我们提出了一种从短寿命脊椎动物模型 Nothobranchius furzeri 的大脑中分离细胞核的方案,用于下游应用,例如单核RNA测序或转座酶可接近染色质测序(ATAC-seq)的单核测定。

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