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1Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, 2Department of Oral and Maxillofacial Surgery,Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Reseah Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 3State Key Laboratory of Natural and Biomimetic Drugs,Peking University
Chapters
- 00:05Introduction
- 00:56Animal Procedures
- 02:43Two-Photon Microscope Set-up
- 03:17Vessel Imaging and Permeability Detection
- 05:01Results: Detecting Endothelial Barrier Function in the Mouse Submandibular Gland
- 06:30Conclusion
Nel presente protocollo, la funzione di barriera endoteliale della ghiandola sottomandibolare (SMG) è stata valutata iniettando diversi traccianti fluorescenti pesati molecolari nelle vene angolari di modelli animali in vivo sotto un microscopio a scansione laser a due fotoni.
